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摘要: |
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以铁皮石斛（Dendrobium catenatum）嫩茎为试验材料，建立组织培养体系。结果表明，铁皮
石斛嫩茎用 HgCl 2 试剂消毒 5~7 min 效果最好，污染率和死亡率极低。最佳诱导培养基为 MS 6-BA1.5
mg/L NAA0.5 mg/L KT0.2 mg/L 3% 蔗糖，诱导率达到 96%。最佳继代培养基是 MS 6-BA1.5 mg/
L NAA0.5 mg/L KT0.2 mg/L 5% 香蕉提取液 5% 水解酪蛋白 3% 蔗糖，增值系数达到 8.0。最适宜铁
皮石斛组培苗生根的培养基为 1/2MS 6-BA0.1 mg/L IBA1.0 mg/L 5% 香蕉提取液 2% 蔗糖，生根率为
97%。移栽 30 d 后，成活率达到 90% 以上。
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关键词:
铁皮石斛;外植体;组织培养
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DOI：
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分类号：
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基金项目： |
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Study on Tissue Culture of Dendrobium catenatum |
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LUO Shaoqiang
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Kui Dong state-owned forest farm of Zhaoqing,Guangdong,Guangning,526332.China
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Abstract: |
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The tender stem segments of Dendrobium candidum was used for tissue culture and micropropagation. The results showed that 0.1% mercuric chloride solution (5-7min) was appropriate, the pollution and mortality rate was low. The best medium for proliferation was MS 6-BA1.5mg/L NAA0.5mg/L KT0.2mg/L 3%S, the induction rate was 96%. The optimum medium for subculture was MS 6-BA1.5mg/L NAA0.5mg/L KT0.2mg/L 5?nana mud 5% Lactium 3%S, the proliferation coefficient is 8.0, The rooting rate of 97%could be achieved in 1/2MS 6-BA0.1mg/L IBA1.0mg/L 5%Bnana mud 2%S.After 30days, the survival rate was more than 90%.
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Key words:
Dendrobium candidum;explants;tissue culture
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