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张水花,刘丽婷,黄小春,游丁生,狄岚,叶金山,李田
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华南农业大学,江西省林业科学院,江西省林业科学院,信丰县铁石口镇海信棕榈花卉专业合作社,江西省林业科学院,江西省林业科学院,江西省林业科学院
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摘要: |
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在含有0.5 mg/L NAA+0.5 mg/L 6BA+60.0 g/L蔗糖+6.0 g/L琼脂的MS培养基上分别添加6种不同浓度的2,4D，对3种不同树龄布迪椰子种子的成熟胚进行愈伤组织诱导。结果表明：以6~7 a生布迪椰子种子成熟胚的诱导愈伤组织效果最好，添加2,4D则以浓度为0.5 mg/L的效果最好。
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关键词:
布迪椰子；胚；愈伤组织；诱导
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DOI：
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分类号：
S792.91
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基金项目：江西省林业科学院青年基金项目“布迪椰子等优良棕榈科植物引种及培育关键技术研究”(2013520802) |
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Study on Callus Inducement of Mature Embryos of Butia capitata Seeds |
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Zhang shuihua,Liu liting,Huang xiaochun,You dingsheng,Di lan,Ye jinshan and Li tian
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Jiangxi Academy of Forestry Nanchang,Jiangxi Academy of Forestry Nanchang,XF-Hisense palm and flowers production cooperatives,Jiangxi Academy of Forestry Nanchang,Jiangxi Academy of Forestry Nanchang,Jiangxi Academy of Forestry Nanchang
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Abstract: |
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Phytohormone 2,4D were applied in inducing callus of mature embryo of Butia capitata seeds in this study. The concentration of 2,4D had six level from 0.5 to 3.0 mg/L. The B. capitata were divided into 3 age groups: 6—7, 40 and 50—60 years old. The MS culture medium consisted of 0.5 mg/L NAA, 0.5 mg/L 6BA, 60.0 g/L sugar and 6.0 g/L agar. The results were as follows: the inducing effect were best in 6—7 years old group, and the optimum concentration of 2,4D was 0.5 mg/L.
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Key words:
Butia capitata；embrgo；callus；induction
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