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朱永钊,曾雷
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广东从化陈禾洞省级自然保护区管理处,1.投注app
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摘要: |
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开展光叶楮（Broussonetia papyrifera）外植体消毒、侧芽诱导、继代增殖和生根诱导试验。以75%乙醇 0.15%升汞消毒外植体，干净外植体获得率达40%；采用MS 6BA 2.0 mg/L NAA 0.5 mg/L培养基，侧芽诱导分化率达80%；采用MS 6BA 1.0 mg/L NAA 0.5 mg/L继代培养，增殖倍数达3.96倍；采用1/2MS IBA1.0 mg/L或GGR6 0.5～1.0 mg/L进行生根培养，瓶苗生根率达100%，形成了可以产业化的光叶楮组织培养技术流程
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关键词:
光叶楮;组织培养
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DOI：
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分类号：
S722.3 7
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基金项目：广东省林业科技创新专项“铁皮石斛、三叶五加和辣木等珍贵药用及食用植物高效培育技术研究”（2012KJCX01501） |
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Study on Tissue Culture Breeding Technology of Broussonetia papyrifera |
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zhuyongzhao and Zeng Lei
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Abstract: |
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The tissue culture technology of Broussonetia papyrifera was studied by explants disinfection, lateral bud induction, subculture multiplication and root induction. Explants were sterilized using 75% ethanol and 0.15% mercuric chloride, the survival rate was 40%. The lateral buds planted in MS medium containg 6BA 2.0 mg/L and NAA 0.5 mg/L showed the highest bud differentiation rate (80%). The optimum medium for subculture was MS 6BA 1.0 mg/L NAA 0.5 mg/L, the proliferation times was 3.96. The rooting rate of 100% could be obtained by using 1/2MS IBA1.0 mg/L or GGR6 0.5~1.0 mg/L. The results of this study formed a high potential of large scale commercial production of B. papyrifera.
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Key words:
Broussonetia;papyrifera, tissue;culture
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