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摘要: |
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初步观测了杜鹃红山茶的2n花粉自然发生情况，证实杜鹃红山茶有未减数花粉的存在，其自然发生比例为0～1.66%。有效诱导花芽大小筛选结果表明，花芽纵轴为18～25 mm时均可起到诱导作用，较为有效的诱导时期为花芽纵轴在22～23 mm之间；最优的2n花粉诱导处理方式为0.5%秋水仙素+2%DMSO棉浸2 d、0.1%秋水仙素+2%DMSO棉浸5 d、1.0%秋水仙素+2%DMSO注射2次、1.0%秋水仙素+2%DMSO注射3次，其平均2n花粉得率分别为16.57%、7.88%、6.62%和6.37%。通过对不同倍性花粉萌发动态观察，发现2n花粉的萌发较正常花粉要滞后1～2 h，花粉管生长也较缓慢，随着2n花粉体积的增大萌发率逐渐降低。
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关键词:
杜鹃红山茶 2n花粉 诱导
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DOI：
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基金项目：国家十一五林业科技支撑项目“优质杜鹃红山茶、吊钟花新品种选育”（2006BAD01A1802）；广东省林业科技创新专项资金项目“杜鹃红山茶、火焰木等木本花卉产业化关键技术研究与示范”（2009KJCX010）；广州市林业科技计划项目“杜鹃红山茶新品种选育”。 |
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Study on 2n pollen under natural condition and induction in Camellia azalea Wei |
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Abstract: |
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Observation of spontaneous occurrence of 2n pollen in Camellia azalea Wei, confirmed the existence of unreduced pollen in them. 2n pollen′s occurrence value 0～1.66%. Effective induction buds screening results showed that the treatments can obtain 2n pollen when the flower buds polar length is 18～25 mm, especially in 22～23 mm is the most effective treatment stage; The best treatments is 0.5%+2%DMSO cotton wraped 2 days, 0.1%+2%DMSO cotton wraped 5 days, 1.0%+2%DMSO injection 2 times and 1.0%+2%DMSO injection 3 times. It′s average 2n pollen proportion were 16.57%、7.88%、6.62% and 6.37%. Through the dynamic observation of different polypoid pollen germination of Camellia azalea Wei, it was found that the germination of 2n pollen later 1～2 hours than n pollen, and it tube growth rate was also lower, the germination rate of 2n pollen getting lower as its size increases.
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Key words:
Camellia azalea Wei 2n pollen Inducement
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